| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tubulin gamma-1 chain;Gamma-1-tubulin;Gamma-tubulin complex component 1;GCP-1;TUBG1;TUBG; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Tubulin gamma |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TUBG1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone DAE-20; isotype Rabbit IgG; reactivity: Human,Monkey,Mouse,Rat,Zebrafish. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-Tubulin gamma Rabbit Monoclonal Antibody catalog # M06313. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat, Zebrafish.
Key elements and design rationale
- Target: TUBG1 (Tubulin gamma-1 chain).
- Antibody format: Monoclonal; clone DAE-20; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Monkey,Mouse,Rat,Zebrafish (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TUBG1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation. Reported cellular localization context: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Tissue expression notes (as provided): Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
Research relevance and current trends
- Research context keywords from the source record include: Cytoskeleton,Cytoskeleton/ECM,Microtubules,Signal Transduction,Tubulin.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate TUBG1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TUBG1 expression by Western blot in cell or tissue lysates, Detect TUBG1 in FFPE tissue sections by immunohistochemistry, Localize TUBG1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify TUBG1-positive cells by flow cytometry in single-cell suspensions, Enrich TUBG1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 42 kDa; calculated MW: 51170 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 42 kDa
- Cellular localization (provided): Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .
- Tissue details (provided): Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
