| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glutaredoxin-3;PKC-interacting cousin of thioredoxin;PICOT;PKC-theta-interacting protein;PKCq-interacting protein;Thioredoxin-like protein 2;GLRX3;PICOT, TXNL2;HUSSY-22; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human TXNL2 recombinant protein (Position: L89-S177). Human TXNL2 shares 93.3% and 94.4% amino acid (aa) sequence identity with mouse and rat TXNL2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes glutaredoxin-3 (gene GLRX3) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 37.4 kDa, and the supplier reports an observed band at about 37 kDa.
Glutaredoxin-3 (GLRX3, also called PICOT or TXNL2) is a cytosolic protein that, with BOLA2, helps deliver [2Fe-2S] clusters to iron-sulfur proteins. It lacks classic thioredoxin activity, is needed for hemoglobin maturation and has been linked to control of cardiac hypertrophy.
| Target | Glutaredoxin-3 (gene GLRX3; UniProt O76003, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 37.4 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
|---|
Samples with a confirmed band (WB): human Jurkat cells, rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TXNL2 fragment (Leu89–Ser177), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.3% and 94.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places glutaredoxin-3 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
