| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosinase;1.14.18.1;LB24-AB;Monophenol monooxygenase;SK29-AB;Tumor rejection antigen AB;TYR; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Tyrosinase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAHE-20 is a rabbit monoclonal antibody that recognizes tyrosinase in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 60.4 kDa, and the supplier reports an observed band at about 50–80 kDa.
Tyrosinase (TYR) is a copper-containing enzyme in melanosomes that catalyzes the rate-limiting steps of melanin synthesis, converting tyrosine to DOPA and DOPA to dopaquinone. It is a key marker of melanocytes and melanoma, and loss-of-function mutations cause oculocutaneous albinism type 1.
| Target | Tyrosinase (gene TYR; UniProt P14679, human) |
|---|---|
| Clone | AAHE-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 60.4 kDa |
| Observed MW | 50–80 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): mouse B16-F10 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human tyrosinase.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places tyrosinase in the melanosome membrane and melanosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
