| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Translocating chain-associated membrane protein 2;TRAM2;KIAA0057; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human UBE4A |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-UBE4A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 26U91; isotype IgG; reactivity: Human. Reported application contexts include WB, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-UBE4A Rabbit Monoclonal Antibody catalog # M11871. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: UBE4A (Translocating chain-associated membrane protein 2).
- Antibody format: Monoclonal; clone 26U91; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
UBE4A (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Necessary for collagen type I synthesis. May couple the activity of the ER Ca (2+) pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca (2+) concentration at the site of collagen synthesis, and a high Ca (2+) concentration may be necessary for the function of molecular chaperones involved in collagen folding. . Reported cellular localization context: Membrane ; Multi-pass membrane protein . Tissue expression notes (as provided): Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. .
Research relevance and current trends
- Research context keywords from the source record include: Organelle Proteins,Protein Trafficking,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate UBE4A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect UBE4A expression by Western blot in cell or tissue lysates, Localize UBE4A by immunofluorescence/immunocytochemistry in cultured cells, Quantify UBE4A-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 123 kDa; calculated MW: 43328 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 123 kDa
- Cellular localization (provided): Membrane ; Multi-pass membrane protein .
- Tissue details (provided): Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
