| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin conjugation factor E4 A; RING-type E3 ubiquitin transferase E4 A; UBE4A; KIAA0126 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human UBE4A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26U91 is a rabbit monoclonal antibody that recognizes ubiquitin conjugation factor E4 A (gene UBE4A) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 122.6 kDa, and the supplier reports an observed band at about 123 kDa.
UBE4A is a U-box ubiquitin ligase that can act as an E3 ligase and may also serve as an E4 factor, lengthening polyubiquitin chains on proteins already ubiquitinated by another ligase. It forms Lys-48-linked chains, which typically target proteins to the proteasome.
| Target | Ubiquitin conjugation factor E4 A (gene UBE4A; UniProt Q14139, human) |
|---|---|
| Clone | 26U91 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 122.6 kDa |
| Observed MW | 123 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human UBE4A.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ubiquitin conjugation factor E4 A in the cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
