| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Antigen KI-67;MKI67; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human UCP1+UCP3 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-Ucp1/3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 18U19; isotype IgG; reactivity: Mouse,Rat. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-UCP1+UCP3 Rabbit Monoclonal Antibody catalog # M00255. Tested in WB, IP applications. This antibody reacts with Mouse, Rat.
Key elements and design rationale
- Target: Ucp1/3 (Antigen KI-67).
- Antibody format: Monoclonal; clone 18U19; isotype IgG.
- Host: Rabbit.
- Species reactivity: Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
Ucp1/3 (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Thought to be required for maintaining cell proliferation. Reported cellular localization context: Nucleus . Nucleus, nucleolus . Chromosome . Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. Tissue expression notes (as provided): Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). .
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cell Biology,Cell Cycle,Cell Division,Cell Type Marker,Cell Type Markers,Neurogenesis,Neurology Process,Neuron Marker,Neuroscience,Tags & Cell Markers,Tumor Biomarkers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate Ucp1/3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect Ucp1/3 expression by Western blot in cell or tissue lysates, Enrich Ucp1/3 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 34 kDa; calculated MW: 358694 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 34 kDa
- Cellular localization (provided): Nucleus . Nucleus, nucleolus . Chromosome . Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
- Tissue details (provided): Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
