Anti-UMOD Antibody Picoband®

Overview

Anti-UMOD Antibody Picoband® is an antibody for UMOD detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: UMOD (collagen, type XVIII, alpha 1); UniProt: P07911
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 90-110 kDa, calculated 70 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-UMOD Antibody Picoband® catalog # A01303-2.

Biological background

Biological context: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.

Expression and localization notes: cellular localization: Secreted, extracellular space, extracellular matrix., tissue context: Present in multiple organs with highest levels in liver, lung and kidney..

Common research applications

• Western blotting (WB): Compare UMOD levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of UMOD in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify UMOD-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Uromodulin (UMOD), also known as Tamm–Horsfall protein (THP), is a glycoprotein that in humans is encoded by the UMOD gene. The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Secreted, extracellular space, extracellular matrix.
• Tissue details: Present in multiple organs with highest levels in liver, lung and kidney.
• Research category: Angiogenesis,Cancer,Cardiovascular,Collagen,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.