| Field | Specification |
|---|---|
| Target | |
| Alternative names | Utrophin; Dystrophin-related protein 1; DRP-1; UTRN; DMDL; DRP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Utrophin/UTRN recombinant protein (Position: L1211-K2294). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets utrophin (gene UTRN) in human and mouse samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 394.5 kDa, and the supplier reports an observed band at about 394 kDa.
Utrophin is a large cytoskeletal protein related to dystrophin. It links actin to the dystrophin-associated glycoprotein complex at the cell membrane and is found at neuromuscular junctions and in many tissues. Because it can partly substitute for dystrophin, utrophin is studied in muscle biology and Duchenne muscular dystrophy.
| Target | Utrophin (gene UTRN; UniProt P46939, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 394.5 kDa |
| Observed MW | 394 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Sw620 cells, human Caco-2 cells, human THP-1 cells, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Immunogen
Recombinant human Utrophin/UTRN fragment (Leu1211–Lys2294), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places utrophin in the postsynaptic cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Isoform 1 has high expression in muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
