| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | V1bR, AVPR V1b, AVPR V3, Antidiuretic hormone receptor 1b, Arginine vasopressin receptor 1B, Vasopressin V3 receptor |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
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| Target |
Overview
Anti-Vasopressin V1B Receptor (AVPR1B) Antibody is an antibody targeting V1bR, AVPR V1b, AVPR V3, Antidiuretic hormone receptor 1b, Arginine vasopressin receptor 1B, Vasopressin V3 receptor Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: V1bR, AVPR V1b, AVPR V3, Antidiuretic hormone receptor 1b, Arginine vasopressin receptor 1B, Vasopressin V3 receptor (also reported as V1bR, AVPR V1b, AVPR V3, Antidiuretic hormone receptor 1b, Arginine vasopressin receptor 1B, Vasopressin V3 receptor).
- Immunogen/epitope region: Intracellular, C-terminus.
- Homology note: Mouse - 12/14 amino acid residues identical; human - not recommended for use with human sample (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Specificity statement (as provided): Not recommended for human samples..
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Vasopressin V1b receptor (also called V3R) is a G protein-coupled receptor (GPCR) of the Gq type, thus activating the phospholipase C signaling pathway upon activation. It is part of a family of GPCRs triggered by vasopressin that further include V1R, V2R, oxytocin receptors and purinergic receptors. While V1R and V3R are Gq GPCRs, V2R is coupled to the Gs protein and activates adenylate cyclase1,2.The V1b receptor has an intracellular C-terminal, seven transmembrane domains, and an extracellular N-tail; the transmembrane and extracellular domains are responsible for interacting with agonists and to signal for activation of the coupled Gq receptor1,2.The V1b receptor is mainly detected in the corticotrophs of the anterior pituitary gland in the brain where it regulates the release of adrenocorticotrophic hormone (ACTH) upon stimulation of vasopressin-corticotrophin-releasing hormone conjugates2.V1b KO mice show that the receptor is critical for the functionality of the hypothalamic-pituitary-adrenal axis, and demonstrate its importance in regulating glucose levels.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-VR011.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-VR011; Negative control: BLP-VR011.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
