| Field | Specification |
|---|---|
| Target | |
| Alternative names | Voltage-dependent anion-selective channel protein 1;VDAC-1;hVDAC1;Outer mitochondrial membrane protein porin 1;Plasmalemmal porin;Porin 31HL;Porin 31HM;VDAC1;VDAC; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human VDAC/Porin, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets non-selective voltage-gated ion channel VDAC1 in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 30.8 kDa, and the supplier reports an observed band at about 35 kDa.
VDAC1 is the most abundant voltage-dependent anion channel of the mitochondrial outer membrane. It forms a pore that lets ions and metabolites such as ATP, ADP and pyruvate pass between the cytosol and mitochondria. VDAC1 is also implicated in apoptosis and is a common mitochondrial outer-membrane marker.
| Target | Non-selective voltage-gated ion channel VDAC1 (gene VDAC1; UniProt P21796, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 30.8 kDa |
| Observed MW | 35 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human Caco-2 cells, human A549 cells, human 293T cells, human HepG2 cells, rat heart tissue, rat kidney tissue, mouse heart tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse cardiac muscle tissue, rat cardiac muscle tissue, human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human VDAC/Porin. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places non-selective voltage-gated ion channel VDAC1 in the mitochondrion outer membrane, cell membrane and membrane raft. Tissue expression noted by UniProt (human): Expressed in erythrocytes (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hong Song et al. (2021) Chronic hypoxia alters cardiac mitochondrial complex protein expression and activity in fetal guinea pigs in a sex-selective manner. American Journal of Physiology-Regulatory Integrative and Comparative Physiology. 10.1152/ajpregu.00004.2021
Quebedeaux Tabitha M. et al. (2021) Chronic Hypoxia Inhibits Respiratory Complex IV Activity and Disrupts Mitochondrial Dynamics in the Fetal Guinea Pig Forebrain. Reproductive Sciences. 10.1007/s43032-021-00779-w
Xiaobing Hong et al. (2018) High molecular weight fibroblast growth factor 2 induces apoptosis by interacting with complement component 1 Q subcomponent–binding protein in vitro. Journal of Cellular Biochemistry. 10.1002/jcb.27131
Shaoqing Li et al. (2021) Nicorandil, an ATP-sensitive potassium channel activation, attenuates myocardial injury in rats with ischemic cardiomyopathy. Medical Molecular Morphology. 10.1007/s00795-021-00306-5
