| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular endothelial growth factor D;VEGF-D;c-Fos-induced growth factor;FIGF;FIGF;VEGFD; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human VEGFD recombinant protein (Position: F89-R205). Human VEGFD shares 94% and 94.9% amino acid (aa) sequence identity with mouse and rat VEGFD, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets vascular endothelial growth factor D (gene VEGFD) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 40.4 kDa, and the supplier reports an observed band at about 38 kDa.
VEGF-D is a growth factor of the VEGF family that binds and activates VEGFR-2 and VEGFR-3. It promotes the growth of blood and lymphatic vessels, with a particular role in lymphangiogenesis, and it is studied in tumor lymphatic spread and in vascular development.
| Target | Vascular endothelial growth factor D (gene VEGFD; UniProt O43915, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 40.4 kDa |
| Observed MW | 38 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): WB: rat heart tissue, mouse lung tissue, mouse kidney tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human VEGFD fragment (Phe89–Arg205), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 94% and 94.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes vascular endothelial growth factor D as a secreted protein. Tissue expression noted by UniProt (human): Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Du Li-Cheng et al. (2014) VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma. Reproductive Biology and Endocrinology. 10.1186/1477-7827-12-14
Dong Shengxiang et al. (2009) Efficacy and safety of 32P-nanocolloid for treatment of distant lymph node metastasis in VX2 tumor-bearing rabbits. Annals of Nuclear Medicine. 10.1007/s12149-008-0188-2
