| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vinculin ;Metavinculin ;Vcl ; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Human vinculin, purified from uterus. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone hVIN-1 is a mouse monoclonal antibody that recognizes vinculin (gene Vcl) in chicken, human, monkey, mouse and rat samples and is validated for Western blot, IHC and ICC/IF.
Vinculin is an actin-binding protein found at focal adhesions and adherens junctions. It links integrin- and cadherin-based adhesions to the actin cytoskeleton and helps these junctions respond to mechanical force. Vinculin is a standard marker of focal adhesions in studies of cell adhesion, migration and mechanobiology.
| Target | Vinculin (gene Vcl; UniProt P85972, rat) |
|---|---|
| Clone | hVIN-1 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Chicken, Human, Monkey, Mouse, Rat |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | Mouse ascites fluid; 1.2% sodium acetate; 2 mg BSA; with 0.01 mg NaN3 as preservative |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1–2 µg/mL (Human, Chicken, Monkey, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Immunohistochemistry | Not specified by the supplier |
Immunogen
Human vinculin, purified from uterus.
Reactivity Notes
The supplier lists reactivity with chicken, human, monkey, mouse and rat. UniProt places vinculin in the cell membrane, cell junction and cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xinyi Li et al. (2026) Biomimetic hydrogel strategy inspired by sea cucumbers: Integrating antibacterial, osteoimmune, and osteogenic functions for infected bone repair. Biomaterials. 10.1016/j.biomaterials.2026.124080
Yujia Han et al. (2025) Biodegradable Magnesium alloy Janus membrane with surface-selective osteoinduction and soft tissue healing properties in guided bone regeneration. Acta Biomaterialia. 10.1016/j.actbio.2025.02.001
AslemarzAzam et al. (2024) An EpCAM/Trop2 mechanostat differentially regulates collective behaviour of human carcinoma cells. EMBO Journal. 10.1038/s44318-024-00309-9
Manna Wang et al. (2020) Mechanical Ring Interfaces between Adherens Junction and Contractile Actomyosin to Coordinate Entotic Cell-in-Cell Formation. Cell Reports. 10.1016/j.celrep.2020.108071
Qian Yao et al. (2017) LRP6 promotes invasion and metastasis of colorectal cancer through cytoskeleton dynamics. Oncotarget. 10.18632/oncotarget.22759
Zhou Hai-Hong et al. (2019) Erastin Reverses ABCB1-Mediated Docetaxel Resistance in Ovarian Cancer. Frontiers in Oncology. 10.3389/fonc.2019.01398
Nan Xing-Wei et al. (2019) Survivin Promotes Piperlongumine Resistance in Ovarian Cancer. Frontiers in Oncology. 10.3389/fonc.2019.01345
Wang Kun et al. (2019) Targeting uPAR by CRISPR/Cas9 System Attenuates Cancer Malignancy and Multidrug Resistance. Frontiers in Oncology. 10.3389/fonc.2019.00080
Yuan Meng-Ling et al. (2018) Inhibition of WEE1 Suppresses the Tumor Growth in Laryngeal Squamous Cell Carcinoma. Frontiers in Pharmacology. 10.3389/fphar.2018.01041
Wu Chengsheng et al. (2025) Targeting pancreatic cancer cell stemness by blocking fibronectin-binding integrins on cancer-associated fibroblasts. Cancer Research Communications. 10.1158/2767-9764.CRC-24-0491
