| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Pantetheinase;3.5.1.92 ;Pantetheine hydrolase;Tiff66;Vascular non-inflammatory molecule 1;Vanin-1;VNN1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human VNN1 recombinant protein (Position: Q22-K192). Human VNN1 shares 82.2% amino acid (aa) sequence identity with mouse VNN1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of VNN1 (Pantetheinase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-VNN1 Antibody Picoband® catalog # PB10106. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human VNN1 recombinant protein (Position: Q22-K192). Human VNN1 shares 82.2% amino acid (aa) sequence identity with mouse VNN1. (reported region: Q22-K192).
- Molecular weight context: reported MW: 57 kDa; calculated MW: 57012 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Pantetheinase; Pantetheinase. Pantetheinase is an enzyme that in humans is encoded by the VNN1 gene. This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24. Functional note: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. . Reported localization: Cell membrane ; Lipid-anchor, GPI-anchor . Expression/tissue context: Widely expressed with higher expression in spleen, kidney and blood. Overexpressed in lesional psoriatic skin. .
Research relevance and current trends
- Cardiovascular: Researchers commonly examine how VNN1 (Pantetheinase) relates to this theme using model systems and orthogonal readouts.
- Cofactors: Researchers commonly examine how VNN1 (Pantetheinase) relates to this theme using model systems and orthogonal readouts.
- Vitamins/Minerals: Researchers commonly examine how VNN1 (Pantetheinase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative VNN1 (Pantetheinase) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
