| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pantetheinase;3.5.1.92 ;Pantetheine hydrolase;Tiff66;Vascular non-inflammatory molecule 1;Vanin-1;VNN1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human VNN1 recombinant protein (Position: Q22-K192). Human VNN1 shares 82.2% amino acid (aa) sequence identity with mouse VNN1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects pantetheinase (gene VNN1) in human samples and is validated for Western blot. The predicted molecular weight is 57.0 kDa, and the supplier reports an observed band at about 57 kDa.
Vanin-1 (VNN1) is a GPI-anchored pantetheinase that splits pantetheine into pantothenic acid (vitamin B5) and cysteamine. It thereby recycles vitamin B5 for coenzyme A synthesis and releases cysteamine, which affects cellular redox balance. Vanin-1 is studied in oxidative stress, inflammation and metabolism.
| Target | Pantetheinase (gene VNN1; UniProt O95497, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 57.0 kDa |
| Observed MW | 57 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): SKOV3 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human VNN1 fragment (Gln22–Lys192), expressed in E. coli. Sequence identity with the mouse ortholog: 82.2%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places pantetheinase in the cell membrane. Tissue expression noted by UniProt (human): Widely expressed with higher expression in spleen, kidney and blood. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
