| Field | Specification |
|---|---|
| Target | |
| Alternative names | von Willebrand factor;vWF;von Willebrand antigen 2;von Willebrand antigen II;Vwf ; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse VWF recombinant protein (Position: M1304-E1452). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes von Willebrand factor (gene Vwf) in mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 309.3 kDa, and the supplier reports an observed band at about 309 kDa.
Von Willebrand factor (VWF) is a large multimeric plasma glycoprotein made by endothelial cells and megakaryocytes. At sites of vessel injury it bridges exposed collagen to the platelet receptor GPIb, supporting platelet adhesion, and it carries and protects coagulation factor VIII. Its deficiency causes von Willebrand disease, the most common inherited bleeding disorder.
| Target | Von Willebrand factor (gene Vwf; UniProt Q8CIZ8, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 309.3 kDa |
| Observed MW | 309 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Rat) |
Samples with a confirmed band (WB): mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): mouse liver tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): rat liver tissue, rat lung tissue.
Immunogen
Recombinant mouse VWF fragment (Met1304–Glu1452), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes von Willebrand factor as a secreted protein. Tissue expression noted by UniProt (mouse): Plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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