| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transient receptor potential cation channel subfamily V member 2; TrpV2; Osm-9-like TRP channel 2; OTRPC2; Vanilloid receptor-like protein 1; VRL-1; VRL |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human VRL1/TRPV2 recombinant protein (Position: S7-K390). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transient receptor potential cation channel subfamily V member 2 (gene TRPV2) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 86.0 kDa, and the supplier reports an observed band at about 95 kDa.
TRPV2 is a calcium-permeable, non-selective cation channel of the vanilloid TRP subfamily. Unlike TRPV1 it is not activated by capsaicin, and it can be regulated by growth factors and by mechanical stimuli. It is expressed in immune cells, neurons and muscle and is studied in phagocytosis, mechanosensing and calcium homeostasis.
| Target | Transient receptor potential cation channel subfamily V member 2 (gene TRPV2; UniProt Q9Y5S1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 86.0 kDa |
| Observed MW | 95 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human A431 cells, rat brain tissue, mouse brain tissue, mouse heart tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human VRL1/TRPV2 fragment (Ser7–Lys390), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transient receptor potential cation channel subfamily V member 2 in the cell membrane, cytoplasm and melanosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zheng Ding et al. (2026) TRP channel expression patterns define molecular subtypes, prognosis, and therapeutic targets in gastric cancer. Frontiers in Immunology. 10.3389/fimmu.2026.1752001
Ziqi Zeng et al. (2024) Elevated TRPV2 expression in the facial nerve of rats by cold stimulation: Implications for Bell's palsy. Journal of Stomatology Oral and Maxillofacial Surgery. 10.1016/j.jormas.2024.101895
