| Field | Specification |
|---|---|
| Target | |
| Alternative names | WD repeat-containing protein 1; Actin-interacting protein 1; AIP1; NORI-1; WDR1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human WDR1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29W47 is a rabbit monoclonal antibody that recognizes WD repeat-containing protein 1 (gene WDR1) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 66.2 kDa, and the supplier reports an observed band at about 66 kDa.
WDR1 (AIP1) is an actin-interacting WD repeat protein that works with ADF/cofilin to break down actin filaments. By enhancing cofilin-driven severing, it speeds actin turnover needed for cell migration, cytokinesis and platelet and immune cell function. WDR1 deficiency causes an autoinflammatory disorder.
| Target | WD repeat-containing protein 1 (gene WDR1; UniProt O75083, human) |
|---|---|
| Clone | 29W47 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 66.2 kDa |
| Observed MW | 66 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human WDR1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places WD repeat-containing protein 1 in the cytoplasm, cell projection and cell junction. Tissue expression noted by UniProt (human): Expressed in peripheral blood mononuclear cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
