| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN; DNA helicase, RecQ-like type 3; RecQ protein-like 2; Werner syndrome protein; WRN; RECQ3; RECQL2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human WRN |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24W80 is a rabbit monoclonal antibody that recognizes bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN in human samples and is validated for Western blot. The predicted molecular weight is 162.5 kDa, and the supplier reports an observed band at about 200 kDa.
WRN is a RecQ-family DNA helicase that also has 3′–5′ exonuclease activity, a combination unique among human RecQ helicases. It resolves unusual DNA structures such as stalled replication forks, Holliday junctions and G-quadruplexes, supporting DNA repair, replication and telomere maintenance, and loss-of-function mutations cause Werner syndrome, a premature aging disorder.
| Target | Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN (gene WRN; UniProt Q14191, human) |
|---|---|
| Clone | 24W80 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 162.5 kDa |
| Observed MW | 200 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human K562 cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human WRN.
Reactivity Notes
The supplier lists reactivity with human. UniProt places bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
