| Field | Specification |
|---|---|
| Target | |
| Alternative names | phosphatidylinositol-4,5-bisphosphate 3-kinase; pik3cb |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish PIK3CB recombinant protein (Position: E562-L611). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects phosphatidylinositol-4,5-bisphosphate 3-kinase (gene pik3cb) in zebrafish samples and is validated for IHC and ICC/IF.
PIK3CB encodes p110β, a class IA PI3K catalytic subunit that produces PIP3 and activates AKT signaling. Unlike p110α, it can be activated by G protein-coupled receptors as well as tyrosine kinases, and it is studied in platelet function, insulin signaling and PTEN-deficient tumors.
| Target | Phosphatidylinositol-4,5-bisphosphate 3-kinase (gene pik3cb; UniProt E7F251, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Zebrafish) |
Samples with confirmed staining (IHC): zebrafish spinal cord tissue, zebrafish brain tissue, zebrafish colon tissue, zebrafish testis tissue, zebrafish kidney tissue, zebrafish muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): zebrafish embryo tissue.
Immunogen
Recombinant zebrafish PIK3CB fragment (Glu562–Leu611), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places phosphatidylinositol-4,5-bisphosphate 3-kinase in the cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
