| Field | Specification |
|---|---|
| Target | |
| Alternative names | EC:1.14.16.4; NTPH; TPH 2; Tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived Zebrafish TPH2 recombinant protein (Position: M1-V184). |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects tryptophan 5-hydroxylase 2 (gene tph2) in zebrafish samples and is validated for IHC.
Tryptophan hydroxylase 2 (TPH2) is the neuronal isoform of the enzyme that performs the rate-limiting step of serotonin synthesis, using iron, oxygen and tetrahydrobiopterin to convert L-tryptophan into 5-hydroxytryptophan. It is expressed in serotonergic neurons of the raphe nuclei and is studied in mood, anxiety and sleep research.
| Target | Tryptophan 5-hydroxylase 2 (gene tph2; UniProt A0A8M1P7E6, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
|---|
Samples with confirmed staining (IHC): zebrafish spinal cord tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish TPH2 fragment (Met1–Val184), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
