| Field | Specification |
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| Alternative Names | Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
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Overview
Anti-ZIP10/SLC39A10 (extracellular)-FITC Antibody is an antibody targeting Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10 Polyclonal raised in Rabbit (Fluorescein isothiocyanate (FITC)). This antibody is commonly used in FC, LCI to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10 (also reported as Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10).
- Homology note: Rat – identical. Human – 14 out of 15 amino acid identical. (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis (unlabeled antibody, #AZT-010), and direct flow cytometry (labeled antibody)..
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Fluorescein isothiocyanate (FITC) (may affect detection channel and background).
- Antibody type: Polyclonal / Rabbit IgG.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
ZIP10, also known as Solute Carrier Family 39 Member 10 (SLC39A10), is a zinc transporter protein that belongs to the ZIP (Zrt-, Irt-like Protein) family.1-3 The ZIP family includes 14 members (ZIP1 to ZIP14), that play a critical role in the transport of metal ions, primarily zinc, iron, and manganese, across cell membranes. ZIP family members are primarily involved in the uptake of zinc and iron into the cytoplasm from the extracellular space or from intracellular organelles. Their function mostly increases the cytoplasmic concentration of these metals by transporting them across cellular membranes.1-3 ZIP10 is expressed at the cell membrane and serves as a zinc importer into cells.
Research relevance and current trends
- Linking transporter/channel abundance to ionic homeostasis and excitability-related phenotypes.
- Studying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.
- Combining antibody readouts with functional assays for more complete interpretation.
Common research applications
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: RIC-001-F.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-ZT010; Negative control: RIC-001-F (name: Rabbit IgG Isotype Control-FITC).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
