| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Zinc Transporter 10, Manganese Transporter SLC30A10, Solute carrier family 30 member 10 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-ZnT10 (SLC30A10) Antibody is an antibody targeting Zinc Transporter 10, Manganese Transporter SLC30A10, Solute carrier family 30 member 10 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Zinc Transporter 10, Manganese Transporter SLC30A10, Solute carrier family 30 member 10 (also reported as Zinc Transporter 10, Manganese Transporter SLC30A10, Solute carrier family 30 member 10).
- Immunogen/epitope region: Intracellular, C-terminus..
- Homology note: Mouse - 13 out of 14 amino acid residues identical; Rat - 12 out of 14 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
ZnT10 (also known as SLC30A10) is a member of the Zinc transporter (ZnT) family of proteins that includes 10 members (SLC30A1 to SLC30A10).The SLC30 family reduces the concentration of zinc in the cytoplasm by either exporting it out of the cell or transporting it into intracellular organelles.1ZnT10 was identified as an essential manganese transporter. Manganese is an essential cation for the function of several enzymes, including some crucially important for the metabolism of neurotransmitters and other neuronal metabolic pathways. In fact, mutations in the ZnT10 gene were identified as the underlying cause for hereditary disorders of manganese metabolism leading to hypermanganesemia and accumulation and secondary toxicity in the liver, bone marrow, and the nervous system.2,3ZnT10 also functions as a zinc transporter and can form heterodimers with other ZnT family transporters like ZnT3 and regulate influx of zinc into synaptic vesicles, hence regulating neuronal transmission and plasticity.4
Research relevance and current trends
- Linking transporter/channel abundance to ionic homeostasis and excitability-related phenotypes.
- Studying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.
- Combining antibody readouts with functional assays for more complete interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-ZT020.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-ZT020; Negative control: BLP-ZT020.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
