| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived mouse Apolipoprotein A I recombinant protein (Position: D25-Q264) was used as the immunogen for the APOA1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
APOA1 Antibody / Apolipoprotein A is a anti-APOA1 Mouse antibody Monoclonal (mouse origin) clone 17G5 supplied in Lyophilized format. Recommended for workflows such as ELISA (Cap), Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: APOA1
- Antibody details: Mouse, Monoclonal (mouse origin), clone 17G5, isotype Mouse IgG1
- Format: Lyophilized
- Applications (as listed): ELISA (Cap), WB
Biological background
APOA1 is encoded by the APOA1 gene located on human chromosome 11q23.3. The protein is approximately 243 amino acids long and synthesized mainly in the liver and small intestine. APOA1 circulates in plasma as part of HDL particles, where it activates lecithin-cholesterol acyltransferase (LCAT), an enzyme that esterifies free cholesterol, promoting HDL maturation. It plays a central role in maintaining lipid balance and preventing lipid accumulation in arterial walls.
The APOA1 antibody detects a 28 kilodalton band by western blot and exhibits strong cytoplasmic and extracellular staining in hepatic and intestinal tissues. APOA1 interacts with ATP-binding cassette transporters ABCA1 and ABCG1 to facilitate cholesterol efflux from peripheral cells. It also exhibits antioxidant and anti-inflammatory functions by neutralizing oxidized lipids and modulating cytokine signaling, contributing to cardiovascular protection.
Mutations or decreased expression of APOA1 lead to hypoalphalipoproteinemia and increased risk of coronary artery disease. Conversely, elevated APOA1 levels correlate with improved lipid clearance and reduced atherosclerotic plaque formation. In addition to lipid transport, APOA1 influences glucose metabolism, immune modulation, and amyloid aggregation prevention.
As a multifunctional lipid carrier and protective factor in cardiovascular health, APOA1 serves as both a biomarker and therapeutic target for metabolic disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
