| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human BCAR4. was used as the immunogen for the BCAR4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BCAR4 Antibody / Breast cancer anti-estrogen resistance protein 4 is a anti-BCAR4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: BCAR4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
Functionally, BCAR4 antibody identifies a small regulatory protein and lncRNA complex component that modulates noncanonical Hedgehog signaling. BCAR4 interacts with transcriptional coactivators GLI2 and SMO, promoting activation of target genes linked to proliferation, migration, and survival. Its expression in breast, lung, and cervical carcinomas correlates with tumor aggressiveness and metastatic potential, where it facilitates estrogen-independent growth and resistance to anti-estrogen therapies.
The BCAR4 gene is located on chromosome 16p13.13 and encodes a non-coding transcript that exerts protein-like regulatory effects through RNA-protein interactions. Functionally, BCAR4 acts as a scaffold linking SNIP1 and PNUTS to p300 histone acetyltransferase, thereby enhancing transcriptional activation of downstream effectors involved in cytoskeletal dynamics and metabolic adaptation. In breast cancer, BCAR4-driven signaling enhances glucose metabolism and EMT, supporting invasive phenotypes.
Beyond oncology, BCAR4 is expressed in developmental tissues, including the embryonic brain and reproductive organs, suggesting functions in morphogenesis and differentiation. Experimental silencing of BCAR4 inhibits cell proliferation and migration, underscoring its essential role in oncogenic signaling cascades. Because of its RNA-protein hybrid function, BCAR4 exemplifies a growing class of noncoding RNAs with direct regulatory influence over gene expression.
BCAR4 antibody is widely used in cancer biology, gene regulation, and lncRNA research. It is suitable for immunohistochemistry, immunofluorescence, and western blotting to detect BCAR4 protein expression and localization. This antibody supports investigations into transcriptional signaling, drug resistance mechanisms, and cellular differentiation. In translational research, BCAR4 detection assists in studying hormone therapy resistance and metastasis formation.
Structurally, BCAR4 contains regulatory motifs facilitating interaction with transcriptional cofactors and signaling kinases. Its activity is regulated by chromatin context and nuclear-cytoplasmic shuttling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
