| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human BCLAF1 recombinant protein (Position: H293-E901) was used as the immunogen for the BCLAF1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BCLAF1 Antibody / Bcl-2-associated transcription factor 1 is a anti-BCLAF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: BCLAF1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, FACS, ELISA
Biological background
Bcl-2-associated transcription factor 1 localizes predominantly to the nucleus, where it binds chromatin and interacts with splicing and transcription complexes. It is activated by cellular stress and DNA damage, often promoting pro-apoptotic gene expression or enhancing p53-mediated transcriptional programs. The BCLAF1 antibody is used to detect nuclear foci formation following genotoxic stress, a hallmark of its activation in DNA repair pathways.
Beyond apoptosis, Bcl-2-associated transcription factor 1 contributes to mRNA splicing and export. It interacts with RNA-binding proteins and components of the exon junction complex, linking transcriptional regulation to post-transcriptional RNA processing. Through these activities, it modulates the expression of genes involved in development, immune regulation, and cell differentiation. Using the BCLAF1 antibody, scientists can explore how its dual DNA- and RNA-binding capabilities coordinate nuclear responses to environmental stressors.
Dysregulation of BCLAF1 has been associated with cancer, viral infection, and inflammatory disease. Some viruses, such as adenovirus and cytomegalovirus, express proteins that bind and inactivate BCLAF1 to block host apoptosis and favor viral replication. Conversely, elevated expression of Bcl-2-associated transcription factor 1 has been linked to tumor suppression and enhanced sensitivity to chemotherapeutic agents. These opposing effects underscore the complexity of its biological roles. The BCLAF1 antibody supports functional studies designed to dissect these mechanisms in both normal and transformed cells.
The BCLAF1 antibody is applicable in western blotting, immunofluorescence, chromatin immunoprecipitation, and immunohistochemistry. It provides high specificity for the nuclear form of the protein and allows visualization of expression across tissue types. Investigators studying DNA damage signaling, RNA metabolism, or apoptosis can utilize this antibody to identify dynamic changes in protein localization and abundance following genotoxic or apoptotic stimuli.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
