| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human BNIP3L was used as the immunogen for the BNIP3L antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
BNIP3L Antibody / NIX / BCL2/adenovirus E1B 19 kDa-interacting protein 3-like is a anti-BNIP3L Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Mitochondria, nuclear speckles.
Key elements and design rationale
- Target: BNIP3L
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS
Biological background
NIX antibody detects the same 263-amino-acid integral membrane protein localized to the outer mitochondrial membrane. BNIP3L interacts with members of the BCL2 family and contains an LC3-interacting region (LIR) motif that recruits autophagosomes to mitochondria during mitophagy. Under hypoxic conditions, NIX (BNIP3L) expression is upregulated by hypoxia-inducible factor 1-alpha (HIF1A), initiating mitophagy and limiting reactive oxygen species accumulation. This mechanism is vital for maintaining mitochondrial integrity and metabolic adaptation during oxygen deprivation.
The BNIP3L (NIX) gene resides on chromosome 8p21.2 and is expressed in energy-demanding tissues such as heart, skeletal muscle, and brain. Its transcriptional regulation integrates stress and metabolic cues through signaling pathways including HIF1A, FOXO3, and PGC1A, linking mitochondrial turnover with cellular homeostasis and survival responses.
Pathologically, BNIP3L/NIX plays dual roles in determining cell fate: promoting mitophagy and survival under transient stress, but driving apoptosis during sustained hypoxia or mitochondrial damage. Aberrant regulation of this pathway contributes to cardiomyopathy, neurodegeneration, and tumor resistance to therapy. In cancer biology, elevated NIX (BNIP3L) expression enhances mitochondrial clearance, supporting metabolic flexibility within hypoxic tumor microenvironments. Research using BNIP3L antibody and NIX antibody supports investigations in apoptosis, autophagy, and mitochondrial signaling mechanisms.
This antibody is validated for western blotting, immunofluorescence, and flow cytometry to detect mitophagy-related proteins.
Structurally, BCL2/adenovirus E1B 19 kDa-interacting protein 3-like (NIX) contains a C-terminal transmembrane domain for mitochondrial localization, a BH3-like domain mediating interactions with BCL2 family members, and an LIR motif enabling autophagosome docking. This antibody supports exploration of BNIP3L/NIX function in balancing mitophagy and apoptosis during cellular stress.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
