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The C17.2 cell line is a neural progenitor line derived from the mouse cerebellum using retroviral-mediated oncogene transfer with the avian myc gene. It is one of several lines developed to study the differentiation potential of neural progenitor cells, particularly focusing on neuron and glial cell lineages. C17.2 cells exhibit key characteristics of neural progenitors and can differentiate into both neuronal and glial cells under appropriate conditions, making them valuable for studies on neural development, neurogenesis, and gliogenesis.
One defining feature of C17.2 is its potential to differentiate into distinct neural cell types while maintaining mitotic potential, allowing for extended culture and experimental manipulation. This line expresses markers characteristic of neural stem and progenitor cells and can be induced to express lineage-specific markers depending on the differentiation protocol. The stability and multipotency of C17.2 enable its use in examining factors affecting lineage commitment in neural cells, as well as its application in neural repair and regeneration research.
Researchers employ C17.2 cells in both in vitro and in vivo contexts to understand mechanisms controlling cell fate within the central nervous system (CNS). In addition, the line’s well-characterized gene integration sites and consistent expression of specific neural markers make it a reliable model for neurodevelopmental studies and for exploring the potential therapeutic roles of neural progenitor cells in neurodegenerative disease models.
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Oncogenes: Transformant: v-Myc
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 2 to 4 x 104 cells/cm2
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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