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The CADO-ES1 cell line was established from a malignant pleural effusion taken from a 19-year-old female patient diagnosed with Ewing's sarcoma, primarily located in the right buttock with multiple lung metastases. This cell line provides a valuable tool for research in sarcoma biology, particularly in studying the metastatic processes associated with Ewing's sarcoma. As a disease primarily affecting children and young adults, Ewing's sarcoma is characterized by small round cells that are highly malignant, often exhibiting aggressive behavior and poor prognosis, particularly when metastatic.
Uniquely, CADO-ES1 cells exhibit several critical features valuable for in-depth cancer research. They are heterotransplantable, meaning they can be transplanted into a different species (e.g., mice), which is vital for in vivo studies. This capacity makes them a robust model for studying tumor growth and metastasis in a controlled, yet biologically relevant system. Additionally, these cells have shown the ability to grow independently of anchorage, a characteristic typical of many cancerous cells which allows them to thrive without adhering to the extracellular matrix. Furthermore, CADO-ES1 cells can differentiate neurally in response to cyclic AMP (cAMP), providing a unique perspective into the cellular behaviors influenced by signaling pathways in cancer progression and differentiation.
This combination of features makes CADO-ES1 a significant model for not only understanding the pathology of Ewing's sarcoma but also for the development and testing of targeted therapies that might inhibit the growth and spread of similar cancers. Research utilizing this cell line can contribute to a deeper understanding of cancer cell behavior, metastatic mechanisms, and potential therapeutic targets in sarcomas.
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Receptors expressed: CD99 (Eun Jung Lee, 2003)
- cultureMedium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: Every 3 to 4 days
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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