| Field | Specification |
|---|---|
| Mfr No | |
| Species |
Cal 27 cells is a human squamous cell carcinoma cell line derived from a primary tumor located in the tongue of a 56-year-old male in 1982. Cal 27 cells are epithelial in morphology and are widely used in scientific research to study oral carcinogenesis, the biology of squamous cell and oropharyngeal carcinoma, and to evaluate potential therapeutic agents for head and neck cancers.
The Cal27 cell line has been employed in a variety of research applications, including studies on cell proliferation, apoptosis, particularly in the context of anticancer drug sensitivity and the search for novel anticancer agents, migration, and invasion. They have also been used to investigate the effects of various chemotherapeutic agents such as Cisplatin, radiation therapy, and targeted therapies.
The Cal-27 adenosquamous carcinoma cell line is further used as xenografts, which are instrumental for studying tumor angiogenesis, lymph node metastasis, as well as metastasis and chemoresistance mechanisms. The interaction of Cal27 cells with integrins α6β4 and αvβ3 is of interest, as these molecules play a crucial role in cell adhesion. Studies have explored the effects of targeting these pathways with drugs like vismodegib and itraconazole, substances known to modulate the hedgehog pathway.
Overall, the Cal 27 cell line serves as a robust model for investigating the complex biology of oral squamous cell carcinomas and for testing new therapeutic interventions, thereby contributing to advancements in the management and treatment of oral cancers.
—
Tumorigenic: Yes
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
10%
OFF
15%OFF
20%
OFF
$50
OFF
FREE
SAMPLE
