| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CARD10 recombinant protein (Position: E63-R1014) was used as the immunogen for the CARD10 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CARD10 Antibody / Caspase recruitment domain-containing protein 10 is a anti-CARD10 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: CARD10
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
CARD10 operates as a component of the CARD10-BCL10-MALT1 (CBM) signaling complex. Upon activation of upstream receptors such as GPCRs or EGFR, CARD10 recruits BCL10 and MALT1, initiating downstream IKK complex activation and NF-kappaB signaling. This pathway leads to transcription of genes involved in immune response, cell adhesion, and proliferation. Co-localization studies show CARD10 forming punctate cytoplasmic complexes at the plasma membrane following receptor stimulation.
Structurally, CARD10 contains an N-terminal CARD domain that mediates homotypic interactions with other CARD-containing proteins, a coiled-coil domain for oligomerization, and a C-terminal MAGUK-like domain that anchors it to membranes. It belongs to the CARMA protein subfamily, which also includes CARD11 (CARMA1) and CARD14 (CARMA2), each mediating tissue-specific NF-kappaB activation. Known interaction partners include BCL10, MALT1, and IKKgamma, forming the CBM signalosome responsible for transcriptional activation of inflammatory genes.
Functionally, CARD10 plays a central role in signal transduction from GPCRs to NF-kappaB and JNK pathways. It regulates cytokine production, apoptosis resistance, and epithelial barrier integrity. In epithelial cells, CARD10-mediated signaling contributes to immune surveillance and tissue remodeling, while in vascular endothelial cells, it supports cytokine-induced adhesion molecule expression. CARD10 also regulates responses to hormones, growth factors, and stress stimuli through activation of MAPK and PI3K pathways.
Dysregulation of CARD10 signaling contributes to cancer, chronic inflammation, and cardiovascular diseases. Overexpression enhances NF-kappaB-dependent transcription, promoting tumor growth and resistance to apoptosis. Mutations or altered expression of CARD10 have been reported in colorectal, breast, and renal cancers. Pathway associations include NF-kappaB signaling, GPCR signaling, and innate immune regulation. Developmentally, CARD10 expression is detected in epithelial progenitors and contributes to tissue differentiation.
The CARD10 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
