CCRF-CEM-C7 cell

The CCRF-CEM-C7 cell line is a clone derived from the parent CCRF-CEM cell line, which itself originates from a human acute lymphoblastic leukemia (ALL) of the T-cell type. This cell line was established from peripheral blood taken from a 4-year-old female patient with ALL. The CCRF-CEM-C7 cell line is extensively used in biomedical research, particularly in studies related to cancer biology, drug screening, and mechanisms of chemotherapy resistance. CCRF-CEM-C7 cells are characterized by their robust growth in vitro and are commonly used to assess the cytotoxicity of anti-cancer compounds. These cells express several key markers of T-cell development and are often utilized to investigate T-cell leukemia pathogenesis, T-cell signaling pathways, and the cellular responses to DNA damage. The line has also been important in studies investigating the role of apoptosis in cancer cells, making it a valuable resource for understanding the mechanisms of programmed cell death in response to therapeutic agents. Given its origin and characteristics, CCRF-CEM-C7 serves as a model system for T-cell acute lymphoblastic leukemia, providing insights into the biological behavior of this malignancy and offering a platform for testing therapeutic strategies targeting cellular pathways specific to T-cell malignancies.

SKU:BHC11101069

• SDS (78F0F849D28440588B2823DE0161170E)
• Product Sheet (78F0F849D28440588B2823DE0161170E)
• CCRF-CEM-C7 300398-122 Cytion CoA (PDF)

• cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
• supplements: Supplement the medium with 10% FBS
• dissociationReagent: Accutase
• subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
• freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.