CD25 Antibody

SKU:BHA17130786
Suppliers
NSJ Bioreagents
NSJ Bioreagents
Details Products
Overview
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Anti-CD25 antibody from Rabbit (clone IL2RA/4375R), supplied as purified. Recommended for IHC-P in Human samples.
Target CD25
Clone Number IL2RA/4375R
Conjugate(s) Unconjugated
Host Rabbit
Reactivity Human
Application IHC-P
Options selector
Catalog no. Formulation Size
V4086-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
V4086SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Formulation (2) - 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide, 1 mg/ml in 1X PBS; BSA free, sodium azide free; Size (2) - 100 ug, 20 ug
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Aliquot the CD25 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No V4086
Clonality
  • Recombinant Rabbit Monoclonal
Host Rabbit
Immunogen A recombinant partial protein (within amino acids 1-250) from the human protein was used as the immunogen for the CD25 antibody.
Isotype
  • Rabbit IgG
  • kappa
Product Type
  • Antibodies
  • Primary Antibodies
Purity Protein A/G affinity
Reactivity
  • Human
Storage Aliquot the CD25 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Target CD25
UniProt # P01589

Overview

CD25 Antibody is an antibody targeting CD25. Host: Rabbit. Monoclonal. Clone: IL2RA/4375R. Format: Purified. Applications: IHC-P.

Key elements and design rationale

  • Target: CD25.
  • Immunogen: A recombinant partial protein (within amino acids 1-250) from the human protein was used as the immunogen for the CD25….
  • Species reactivity: Human.
  • Localization: Cell surface,.
  • Conjugate: Unconjugated (no label listed).

Biological background (from provided description)

CD25, or interleukin 2 (IL2) receptor alpha (IL2RA), and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (CD25) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor.

Common research applications

  • IHC: tissue distribution assessment; interpret with matched controls.

Notes for experimental interpretation

  • Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
  • Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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