CD31 Antibody / PECAM-1

Overview

CD31 Antibody / PECAM-1 is an antibody targeting PECAM-1, raised in Goat for protein detection and localization studies where these specifications are required.

Key elements and design rationale

• Target: PECAM-1.
• Antibody identity: Polyclonal (goat origin); Goat Ig.
• Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
• Format: Antigen affinity purified.
• Species reactivity: Human.
• Listed applications: IF, IHC-P, ELISA (peptide) (refer to on-page specifications for application-specific guidance).

Biological background

CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a transmembrane glycoprotein that is found on the surface of various cell types, including platelets, endothelial cells, and leukocytes. One of the key roles of CD31 is its involvement in leukocyte trafficking and adhesion. Studies have shown that CD31 plays a crucial role in regulating the migration of immune cells to sites of inflammation or infection. By interacting with other proteins on the surface of immune cells, CD31 helps to mediate the adhesion and transmigration of these cells across the endothelium, allowing them to reach their target tissues and carry out their immune functions. CD31 has also been shown to regulate other important immune responses. For example, CD31 has been implicated in the modulation of T cell activation and proliferation. By interacting with other signaling molecules, CD31 can influence the signaling pathways that control T cell activation, ultimately shaping the immune response to pathogens or foreign antigens. Additionally, CD31 plays a role in the maintenance of vascular integrity. By promoting interactions between endothelial cells, CD31 helps to maintain the tight junctions that form the blood-brain barrier and other vascular structures. This function of CD31 is crucial for preventing the entry of pathogens and toxins into the bloodstream, protecting the body from potential harm.

Research relevance and current trends

• Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
• Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
• Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.

Common research applications

• Immunofluorescence: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
• Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
• ELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.

Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.

Notes for experimental interpretation

• Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
• Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
• Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
• Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.