| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human CHD4 was used as the immunogen for the CHD4 antibody. |
| Isotype | |
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| Purity | |
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Overview
CHD4 Antibody / Chromodomain-helicase-DNA-binding protein 4 / Mi2-beta is a anti-CHD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: CHD4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
As a key nuclear regulator, CHD4 modulates chromatin accessibility and transcriptional silencing by repositioning nucleosomes and recruiting histone-modifying enzymes. It interacts with co-repressors, including MTA1-3 and HDAC1/2, within the NuRD complex to establish repressive chromatin states. CHD4's helicase and chromodomain motifs enable recognition of methylated histones and remodeling of nucleosomal DNA, linking epigenetic signals to transcriptional control. It functions in diverse pathways such as cell cycle progression, stem cell maintenance, and DNA damage repair.
CHD4 antibody identifies a protein that interacts with PARP1 and BRCA1 at sites of DNA double-strand breaks, coordinating chromatin remodeling and homologous recombination. Through this association, CHD4 contributes to the DNA damage response (DDR), facilitating repair factor recruitment and checkpoint activation. Mutations or misregulation of CHD4 impair genomic stability and can lead to carcinogenesis or developmental disorders. CHD4 also associates with replication protein A (RPA) and proliferating cell nuclear antigen (PCNA), supporting chromatin reassembly during DNA synthesis.
Structurally, CHD4 contains two N-terminal plant homeodomain (PHD) zinc fingers, tandem chromodomains for methylated histone recognition, an ATPase/helicase domain for chromatin remodeling, and a C-terminal region mediating protein-protein interactions. This modular structure allows CHD4 to integrate epigenetic marks with mechanical chromatin rearrangement. It is part of the CHD family of SNF2-like ATPases that also includes CHD3 and CHD5, all involved in transcriptional regulation and chromatin remodeling.
Dysfunction of CHD4 is linked to various diseases. Somatic mutations have been identified in endometrial carcinoma, chronic lymphocytic leukemia, and glioblastoma. Germline CHD4 mutations cause Sifrim-Hitz-Weiss syndrome, characterized by developmental delay, craniofacial abnormalities, and cardiac defects. Pathway analysis places CHD4 in the chromatin remodeling, DNA repair, and cell cycle regulation pathways. It also co-localizes with HDAC1/2 and MTA proteins in nuclear repressor complexes, reinforcing its central role in chromatin dynamics.
Immunohistochemical staining using CHD4 antibody shows strong nuclear localization in proliferating and stem-like cells. The CHD4 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
