CHO-CXCR7 cell

SKU:BHC11101483
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Overview
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CHO-CXCR7 cell is a cell line (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Monolayer, adherent, Epithelial-like. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Chinese hamster
Morphology Epithelial-like
Growth Properties Monolayer, adherent
Tissue Ovary
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Catalog no. Size
305412L 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305412L
Species Chinese hamster
Disclaimer: The prices displayed for cell lines are exclusively for not-for-profit customers. If you represent a commercial entity, please contact us for alternative pricing.The CHO-CXCR7-Low cell line is a stable recombinant CHO (Chinese Hamster Ovary) cell line expressing the CXCR7 receptor at a low level. This cell line was generated using an innovative landing pad technology, allowing for reproducible and precise integration of the CXCR7 gene at a validated genomic locus. CXCR7, also known as ACKR3, is an atypical chemokine receptor involved in various biological processes, including immune modulation and tumor biology. Unlike classical GPCRs, CXCR7 does not signal through G proteins but instead modulates cellular responses by scavenging chemokines, such as CXCL12 and CXCL11, and interacting with CXCR4 through heterodimer formation. Overexpression of CXCR7 has been implicated in several cancers, including breast, lung, and prostate, where it is associated with enhanced tumor progression, metastasis, and poor prognosis. CXCR7 contributes to cancer progression by altering the tumor microenvironment, promoting angiogenesis, and facilitating cancer cell migration and invasion. Due to its significant role in cancer biology, CXCR7 is an important target in oncology research. The expression of CXCR7 in this cell line was confirmed using flow cytometry.

SKU:BHC11101483

Receptors expressed: CXCR7 (ACKR3)

  • cultureMedium: Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
  • supplements: Supplement the medium with 10% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL.
  • dissociationReagent: Trypsin-EDTA
  • subculturing: For routine adherent cell culture: Aspirate the old culture medium from the adherent cells, and wash them with PBS to remove any remaining medium. After aspirating the PBS, add the appropriate volume of Trypsin/EDTA solution based on the culture vessel size (e.g., 1 ml for a T25 flask, 3 ml for a T75 flask) and incubate at room temperature or 37°C until the cells detach (5-10 minutes). Monitor detachment under a microscope, and gently tap the vessel if necessary to release the cells. Once detached, add complete medium to inactivate the Trypsin/EDTA, gently resuspend the cells, and transfer an aliquot of the cell suspension into a new culture vessel containing fresh medium. Place the vessel in an incubator set to 37°C with 5% CO2, and change the medium every 2-3 days.
  • fluidRenewal: 2 to 3 times per week
  • postThawRecovery: After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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