| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CRF/CRH was used as the immunogen for the CRH antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CRH Antibody / Corticotropin Releasing Hormone is a anti-CRH Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: CRH
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
Functionally, CRH antibody identifies the peptide precursor prepro-CRH, which undergoes proteolytic cleavage to produce the mature active hormone. CRH binds to G protein-coupled receptors CRHR1 and CRHR2 on pituitary corticotrophs, activating adenylate cyclase and cAMP-dependent signaling pathways that induce ACTH synthesis and secretion. ACTH in turn stimulates cortisol release from the adrenal cortex, completing the neuroendocrine feedback loop that maintains stress adaptation and homeostasis.
The CRH gene is located on chromosome 8q13.1 and is primarily expressed in the paraventricular nucleus of the hypothalamus, but also in peripheral tissues such as the placenta, adrenal glands, and gastrointestinal tract. CRH coordinates endocrine, autonomic, and behavioral responses to stress, linking neuroendocrine signaling with emotional and metabolic regulation.
Pathologically, dysregulated CRH expression is associated with anxiety, depression, Cushing�s syndrome, and inflammatory disorders. Overproduction of CRH leads to chronic activation of the HPA axis and elevated cortisol levels, while deficiency impairs stress response and adrenal function. Research using CRH antibody supports studies in neuroendocrinology, stress physiology, and behavioral neuroscience.
CRH antibody is validated for immunohistochemistry, ELISA, and immunofluorescence to detect neuropeptides and hormones.
Structurally, Corticotropin releasing hormone is a 41-residue peptide containing a disulfide bond that stabilizes its helical structure, essential for receptor binding and biological activity. Its precursor includes a signal peptide directing secretion and prohormone regions involved in processing. This antibody enables investigation of CRH's role in HPA axis regulation, neuropeptide signaling, and stress-related disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
