CTLL-2 Cells

CTLL-2, or cytotoxic T lymphocyte cell line-2, is an immortalized mouse cell line that originates from cytotoxic T cells. These cells were obtained through repetitive allogeneic Mixed Tumor-Lymphocyte Cultures (MTLC) of spleen cells from C57BL/6 mice immunized with F4-5 Friend virus (FLV)-induced leukemia cells. This specific derivation makes CTLL-2 a highly relevant model for studying T-cell mediated responses to viral oncogenesis and tumor immunology. The cell line requires the presence of interleukin-2 (IL-2) in its culture medium for survival and proliferation, emphasizing its utility in researching cytokine-driven cell processes. In immunological research, CTLL-2 serves as a critical tool for examining various aspects of T-cell function and cytokine biology. Its dependency on IL-2 for growth and sustenance is particularly useful for exploring the signaling pathways activated by this cytokine, as well as the broader gene expression changes in T cells responding to external stimuli. Furthermore, CTLL-2 is employed in studies related to T-cell receptor (TCR) activation, leading to insights into cell proliferation, apoptosis, and cytokine secretion. These attributes make CTLL-2 essential for high-throughput screening assays aimed at discovering new immunomodulatory agents, and for testing the biological activity of IL-2 formulations, which are pivotal in cancer immunotherapy and autoimmune disease management.

SKU:BHC11100989

• SDS (0192B90A12AA7DB0AE6AB7ACC2FFF787)
• Product Sheet (0192B90A12AA7DB0AE6AB7ACC2FFF787)
• CTLL-2 400482-216SF Cytion CoA (PDF)

• Receptors expressed: IL-2
• Viruses: Tested and found negative for ectromelia virus (mousepox) .
• Karyotype: Not specified

• cultureMedium: i2Cult (We do not supply this product; please consider other suppliers. Please let us know if you need further assistance)
• subculturing: Immediately after thawing, about 50% viable cells were measured using Trypan Blue dye exclusion. The viability of the cells eventually will drop to even lower values. The cell viability should however increase to > 80% within 48 hours, at a cell concentration of about 1 million cells/ml. Subculture the cells at an inoculation density of 40000 cells/ml. Control the cell viability every day. Keep the cells at 37 degree Celsius and 5% CO2.
• seedingDensity: 5 x 105 cells/mL
• fluidRenewal: 2 to 3 times per week
• postThawRecovery: Allow the cells to recover from the freezing process for at least 48 hours.
• freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.