| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Cathepsin F/CTSF was used as the immunogen for the CTSF antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CTSF Antibody / Cathepsin F is a anti-CTSF Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human. Reported localization: Cytoplasm (lysosome).
Key elements and design rationale
- Target: CTSF
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS
Biological background
Functionally, CTSF antibody identifies a 484-amino-acid preproenzyme that undergoes proteolytic maturation into active heavy and light chains. Cathepsin F participates in degradation of intracellular and endocytosed proteins, contributing to antigen presentation via MHC class II molecules. It also influences apoptosis, tissue remodeling, and neurodegenerative processes through regulated proteolysis.
The CTSF gene is located on chromosome 11q13.1 and is expressed in brain, liver, spleen, and macrophages. Cathepsin F maintains lysosomal proteolytic balance and participates in innate immune defense through controlled protein breakdown and turnover.
Pathologically, CTSF mutations cause Kufs disease type B, an adult-onset neuronal ceroid lipofuscinosis characterized by progressive neurodegeneration. Dysregulated Cathepsin F expression has also been associated with cancer progression and inflammatory diseases. Research using CTSF antibody supports studies in lysosomal biology, protease regulation, and neurodegenerative mechanisms.
CTSF antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect lysosomal proteases.
Structurally, Cathepsin F features an N-terminal cystatin-like domain and a papain-like catalytic domain containing the conserved cysteine-histidine-asparagine catalytic triad. This antibody enables examination of CTSF's role in lysosomal degradation and neuronal maintenance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
