| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CXCL13 was used as the immunogen for the CXCL13 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CXCL13 Antibody / C-X-C motif chemokine 13 / BCA1 is a anti-CXCL13 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Flow cytometry (FACS), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human. Reported localization: Cytoplasmic, secreted.
Key elements and design rationale
- Target: CXCL13
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): FACS, IHC, WB
Biological background
Structurally, CXCL13 is a 109-amino-acid secreted protein of approximately 12 kilodaltons that adopts a characteristic chemokine fold stabilized by disulfide bonds. It is expressed by follicular dendritic cells, stromal cells, and certain epithelial and endothelial cells in lymphoid tissues. Upon secretion, CXCL13 establishes chemotactic gradients that attract CXCR5-positive lymphocytes to B cell zones within lymph nodes, spleen, and tertiary lymphoid structures.
The CXCL13 antibody is widely used in immunology, oncology, and infectious disease research to study chemokine signaling, germinal center formation, and immune cell trafficking. Western blot analysis detects a 12 kilodalton band corresponding to CXCL13, while immunohistochemistry reveals extracellular and stromal staining patterns within lymphoid tissues. This antibody supports quantitative and localization studies of B cell chemotactic signaling and lymphoid organogenesis.
Functionally, CXCL13 orchestrates the spatial organization of adaptive immune responses by regulating migration of B cells and T follicular helper cells. Overexpression of CXCL13 is observed in autoimmune diseases such as rheumatoid arthritis, Sjogren's syndrome, and systemic lupus erythematosus, where ectopic lymphoid structures form in inflamed tissues. It also serves as a biomarker for certain cancers and viral infections, reflecting local immune activation. The CXCL13 antibody provides a sensitive and specific reagent for assessing chemokine expression in tissue sections and culture systems.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
