| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human DIO2 was used as the immunogen for the DIO2 antibody. |
| Isotype | |
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Overview
DIO2 Antibody / Type II iodothyronine deiodinase is a anti-DIO2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Rat.
Key elements and design rationale
- Target: DIO2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
Type II iodothyronine deiodinase is a homodimeric membrane-bound enzyme located in the endoplasmic reticulum, characterized by a conserved selenocysteine residue within its catalytic center. It activates prohormone T4 to T3 by inner-ring deiodination, enhancing thyroid hormone receptor activation in target cells. The DIO2 antibody enables visualization of this enzyme in thyroid hormone–responsive tissues, facilitating studies of local hormone activation under varying physiological conditions such as cold exposure, fasting, or inflammation.
Regulation of DIO2 is complex, involving both transcriptional and post-translational control. Its expression increases in response to sympathetic stimulation, especially in brown adipose tissue, where it supports thermogenesis by elevating mitochondrial metabolism. The DIO2 antibody supports research into these adaptive responses, helping elucidate mechanisms that coordinate hormonal signaling and energy expenditure. In the central nervous system, DIO2 provides local T3 for neurons and glial cells, influencing neurodevelopment and hypothalamic feedback regulation of thyroid-stimulating hormone (TSH).
Mutations or polymorphisms in the DIO2 gene have been linked to thyroid disease, insulin resistance, and altered metabolic rate. Abnormal DIO2 activity may contribute to hypothyroidism, obesity, and neuropsychiatric disorders. The DIO2 antibody allows quantification of enzyme expression in tissues and model systems used to study endocrine and metabolic diseases. Detection of Type II iodothyronine deiodinase also aids in assessing how thyroid hormone activation varies among tissues under different metabolic states.
The DIO2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It provides strong cytoplasmic staining consistent with endoplasmic reticulum localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
