| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human DNAJB6 was used as the immunogen for the DNAJB6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
DNAJB6 Antibody / DnaJ homolog subfamily B member 6 is a anti-DNAJB6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: DNAJB6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC
Biological background
DnaJ homolog subfamily B member 6 exists in two major isoforms produced by alternative splicing: a long nuclear/cytoplasmic form (DNAJB6a) and a shorter cytoplasmic form (DNAJB6b). Both isoforms cooperate with Hsp70 to stabilize unfolded or aggregation-prone proteins, particularly under stress conditions such as heat shock or oxidative damage. The DNAJB6 antibody detects these isoforms, allowing analysis of expression patterns and subcellular localization in response to proteotoxic stress.
Mutations in DNAJB6 cause limb-girdle muscular dystrophy type D1 (LGMDD1), a progressive neuromuscular disorder characterized by protein aggregation and muscle weakness. The DNAJB6 antibody is crucial for studying the molecular basis of this disease, enabling quantification of mutant and wild-type protein levels and their aggregation propensity. DnaJ homolog subfamily B member 6 has also been shown to suppress polyglutamine aggregation in Huntington's disease models, highlighting its broader neuroprotective role. The DNAJB6 antibody supports investigations into these mechanisms by providing reliable detection in neuronal and muscle tissues.
Beyond its function in proteostasis, DnaJ homolog subfamily B member 6 participates in transcriptional regulation and signal transduction. It interacts with components of the Wnt/beta-catenin pathway, influencing gene expression and cellular differentiation. The DNAJB6 antibody allows exploration of these noncanonical functions, revealing how chaperones integrate protein quality control with developmental signaling. Elevated expression of DNAJB6 has been associated with enhanced stress resistance and reduced metastasis in some cancer cells, further emphasizing its physiological relevance.
The DNAJB6 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It provides clear cytoplasmic and nuclear localization depending on isoform distribution.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
