DSL-6A-C1 cell

SKU:BHC11100731
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Overview
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DSL-6A-C1 cell is a Acinar cells cell line (Male). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Epithelial-like. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Rat
Disease model Carcinoma, azaserine induced
Morphology Epithelial-like
Growth Properties Adherent
Tissue Pancreas
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Catalog no. Size
500166 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 500166
Species Rat
The DSL-6A/C1 cell line is a pancreatic ductal cell line originally derived from the DSL-6 transplantable acinar cell carcinoma, a tumor established from a primary acinar cell carcinoma of the pancreas in a male Lewis rat. This rat was exposed to azaserine intraperitoneally, leading to the development of the tumor. Initially, upon establishment in culture, DSL-6A/C1 cells retained the capability to produce amylase, a characteristic exocrine enzyme of acinar cells. However, this production ceased within one to two weeks of culture. Over time, as the DSL-6A/C1 cells were maintained in culture and subjected to regrafting experiments, they underwent a notable phenotypic transformation. The cells lost structural and immunohistochemical markers that are typical of acinar cells and instead began expressing markers indicative of ductal cell phenotype. One of the key markers acquired during this transformation is the cystic fibrosis transmembrane regulator (CFTR), which is commonly associated with ductal cells in the pancreas. This shift in marker expression suggests a significant plasticity in the cell line, reflecting changes in cell identity and function that can occur in response to the in vitro environment.

SKU:BHC11100731

Tumorigenic: Yes, in Lewis rats the cells produce solid tumors composed of ductlike structures surrounded by dense fibrous tissue

  • cultureMedium: Waymouth medium (We do not supply this product; please consider other suppliers. Please let us know if you need further assistance)
  • supplements: Supplement the medium with 10% FBS, 2.0 mM L-glutamine
  • dissociationReagent: Accutase
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • seedingDensity: 1 x 104 cells/cm2
  • fluidRenewal: 2 times per week
  • postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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