| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human DVL2 recombinant protein (Position: L89-Y576) was used as the immunogen for the DVL2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DVL2 Antibody / Disheveled segment polarity protein 2 is a anti-DVL2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: DVL2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Upon Wnt ligand binding, DVL2 is recruited to the membrane through Frizzled receptors, where it inhibits the beta-catenin destruction complex composed of Axin, APC, and GSK-3beta. This allows beta-catenin to accumulate and enter the nucleus, where it activates Wnt target genes that regulate growth and differentiation. In addition to its role in the canonical Wnt pathway, DVL2 participates in planar cell polarity signaling, influencing cytoskeletal organization and tissue morphogenesis. Dysregulation of DVL2 expression or phosphorylation disrupts normal Wnt signaling and has been implicated in developmental disorders and multiple cancers, including breast, colon, and lung carcinoma.
The DVL2 antibody is widely used in cell signaling, developmental biology, and oncology to monitor Wnt pathway activation and Disheveled protein dynamics. Western blot analysis identifies multiple DVL2 bands (90-100 kilodaltons) corresponding to phosphorylated isoforms, while immunofluorescence reveals punctate cytoplasmic and membrane-associated staining. This antibody supports investigation of Wnt-mediated transcriptional regulation and cytoskeletal rearrangement. Because DVL2 serves as a convergence point for canonical and non-canonical pathways, it is crucial for studying cell polarity, migration, and tissue patterning.
Beyond development, DVL2 influences cancer progression, stem cell renewal, and inflammation through its regulation of beta-catenin and JNK signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
