| Field | Specification |
|---|---|
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Tissue, milk, and other biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of D-Amino Acids in biological and other samples. The assay uses OD570nm or FL530/585nm for signal readout. Compatible sample input includes Tissue, milk, and other biological samples. Typical stated assay timing is 60 min.
Key elements and design rationale
- Readout format: OD570nm or FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Tissue, milk, and other biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.86 µM (colorimetric assay), 0.18 µM (fluorimetric assay) for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Linear detection range: 0.86 to 500 µM (colorimetric assay) and 0.18 to 50 µM (fluorimetric assay) for 60 min reaction.
Additional feature notes highlight Convenient. The procedure involves adding a single working reagent and reading after 60 minutes; High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to process thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of d-amino acid within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
D-AMINO-ACIDSare not as widespread as their enantiomeric counterparts in proteins but they can be found in organisms ranging from bacteria (cell walls and antibiotics) to mammals (central nervous systems). The presence of D-amino acids in food is also of considerable interest. Racemization of L-amino acids during food processing may affect food quality and nutritional value.BioAssay Systems’ D-amino acid assay uses an enzyme-catalyzed oxidation of D-amino acids to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the D-amino acid concentration in the sample.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.86 µM (colorimetric assay), 0.18 µM (fluorimetric assay).
Procedures and timing
Stated procedure or timing information: 60 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify d-amino acid in tissue, milk by OD570 nm or FL530/585 nm readout.
- Compare treatment or phenotype groups using matched tissue, milk handling.
- Monitor time-course or pre/post changes in tissue, milk across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Will this kit detect L-amino acids?
The DAA enzyme is specific to D-amino acids only.
What samples have you tested?
This kit has been tested on rat brain tissue, mouse brain tissue, cerebrospinal fluid, and dairy products like whole milk.
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol.
How do you use this kit on cerebrospinal fluid?
CSF can be assayed directly and an internal standard should be used.
Samples will need three separate reactions:
1) sample plus standard
2) sample alone
3) sample blank.
For the internal standard prepare 200 µL 1250 µM standard by mixing 125 µL 2 mM Standard and 75 µL dH2O.
For the sample plus standard well, add 5 µL 1250 µM standard and 20 µL sample.
For the sample and sample blank wells, add 5 µL dH2O and 20 µL sample.
Prepare enough Working Reagent (WR) for all assay wells by mixing, for each well, 85 µL Assay Buffer, 1 µL DAA Enzyme, 1 µL HRP Enzyme, 1 µL Dye Reagent. Prepare a WR without the DAA Enzyme for the sample blank well(s).
Add 80 µL of the WR to each well. Tap plate briefly to mix.
Incubate at room temperature for 60 min. Use a plate reader to read OD570nm.
The sample D-amino acid concentration is computed as follows:
where RSAMPLE, RBLANK, and RSTANDARD are the OD or fluorescence values of the Sample, Sample Blank, and the Sample plus Standard respectively. n is the sample dilution factor. Note: The volume of the internal standard is 4× lower than the sample volume; thus, the internal standard concentration should be divided by 4.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
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