| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological samples (e.g. microorganisms, plasma, serum, cultured cells, tissue, and culture media.) |
| Shipping | |
| Species | |
| Storage |
Overview
Hexokinase activity determination in biological samples (e.g. microorganisms, plasma, serum, cultured cells, tissue and culture media.). The assay uses OD565nm for signal readout. Compatible sample input includes Biological samples (e.g. microorganisms, plasma, serum, cultured cells, tissue, and culture media.). Typical stated assay timing is 15 min.
Key elements and design rationale
- Readout format: OD565nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological samples (e.g. microorganisms, plasma, serum, cultured cells, tissue, and culture media.), which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.22 U/L for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Linear detection range (20 μL sample): 0.22 to 100 U/L for 15 min reaction.
Additional feature notes highlight Convenient. Homogeneous “mix-incubate-measure” type assay; High-throughput. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of hexokinase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
HEXOKINASE (E.C. 2.7.1.1)is a mitochondrial and cytosolic enzyme and the first committed step in the glycolysis pathway. Hexose sugars, mainly glucose, are phosphorylated using ATP to form glucose-6-phosphate. Hexokinases are implicated in a myriad of human diseases, including cancer and diabetes. BioAssay Systems’ non-radioactive, colorimetric Hexokinase assay involves a stepwise reaction based on the reduction of the tetrazolium salt MTT in an NADPH-coupled enzymatic reaction to a colored product which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Detection method
Colorimetric (OD 565 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.22 U/L.
Procedures and timing
Stated procedure or timing information: 15 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify hexokinase in microorganisms, plasma, serum by OD565 nm readout.
- Compare treatment or phenotype groups using matched microorganisms, plasma, serum handling.
- Monitor time-course or pre/post changes in microorganisms, plasma, serum across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does this kit supply hexokinase?
No, researchers must supply their own hexokinase enzyme specific to their needs.
Which Hexokinase was used to develop this kit?
A specific isozyme of hexokinase was not used, so the kit should work with any hexokinase. Glucokinase (Hexokinase IV) may require some adjustments as its kinetic parameters are very different from the other isoforms of hexokinase.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
