| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human FBXW8 recombinant protein (Position: M1-V598) was used as the immunogen for the FBXW8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
FBXW8 Antibody / F-box/WD repeat-containing protein 8 is a anti-FBXW8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: FBXW8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, FBXW8 antibody identifies a 616-amino-acid protein that contains an N-terminal F-box motif and multiple C-terminal WD repeats. The F-box domain binds to the SKP1 adaptor protein, anchoring FBXW8 within the SCF complex, while the WD repeats mediate substrate recognition. FBXW8 acts as a specificity factor that determines which proteins are targeted for ubiquitin-dependent degradation. Key substrates include IRS1, TSC2, and cyclin D1, linking FBXW8 to insulin signaling and cell cycle regulation.
The FBXW8 gene is located on chromosome 12q24.31 and encodes a cytoplasmic and nuclear protein expressed in most tissues, particularly in skeletal muscle and liver. FBXW8 associates with the cullin family protein CUL7 to form the CUL7-FBXW8 E3 ligase complex, distinct from classical SCF ligases. This complex regulates growth factor signaling and metabolic homeostasis by mediating the ubiquitination of signaling intermediates involved in mTOR and PI3K pathways.
In physiology, FBXW8 controls insulin receptor signaling by targeting IRS1 for degradation, preventing excessive insulin pathway activation. It also influences neuronal development and differentiation through ubiquitin-mediated turnover of growth-regulating proteins. Disruption of FBXW8 function leads to aberrant signaling, impaired growth, and metabolic imbalance. In cancer, altered FBXW8 expression contributes to tumor cell proliferation and metastasis by deregulating cyclin and kinase levels.
FBXW8 antibody is widely used in cell cycle, signal transduction, and ubiquitin pathway research. It is suitable for immunoblotting, immunoprecipitation, and proteomic studies to identify protein interactions and degradation targets. This antibody provides a valuable tool for investigating the role of F-box proteins in proteostasis and oncogenic signaling. In metabolic studies, FBXW8 detection helps assess the regulation of insulin signaling components.
Structurally, FBXW8's WD-repeat domain forms a beta-propeller structure that enables substrate recognition, while its F-box motif mediates SCF complex assembly. FBXW8 activity is modulated by phosphorylation, ubiquitination, and association with CUL7.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
