| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ethinyl Estradiol; Ethynyl Estradiol; Ethinyl Oestradiol |
| Species | |
| Applications | |
| Assay type | |
| Sample type(s) | Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Shelf life | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This kit quantifies Ethinylestradiol (EE) by competitive ELISA. In this competitive-inhibition assay, analyte in the sample competes with a labeled conjugate for a limited number of antibody binding sites, so signal is inversely proportional to the concentration of Ethinylestradiol (EE) - the standard format for small analytes.
Performance Data
| Assay Type | Competitive ELISA |
|---|---|
| Detection Method | Colorimetric (450 nm, 540-570 nm reference) |
| Detection Range | 31.2-2000pg/mL |
| Sensitivity | 9.48pg/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Species | General |
| Kit Versions | Traditional and Ready-to-use formats |
| Shelf Life | Traditional: 12 months | Ready-to-use: 16 months |
| Manufacturer No. | DL-EE-Ge, DLR-EE-Ge |
| Alternative Names | Ethinyl Estradiol; Ethynyl Estradiol; Ethinyl Oestradiol |
Kit Formats
This assay is offered in two formats: the Traditional kit (12-month shelf life) with standard reagent preparation, and the Ready-to-use kit (16-month shelf life) with pre-prepared components that reduce hands-on setup time. Both formats use the same validated antibody pair and deliver equivalent performance.
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional ELISA kits from DLdevelop, or contact us for related assays and matched reagents for EE research.
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