| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 6-31 from the human protein was used as the immunogen for the Glypican-1 antibody. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Glypican-1 Antibody / GPC1 is an antibody targeting GPC1, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: GPC1.
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Purified.
- Species reactivity: Human.
- Listed applications: WB, IHC-P, FACS (refer to on-page specifications for application-specific guidance).
Biological background
Glypican-1, also known as GPC1, is a glycosylphosphatidylinositol (GPI)-anchored cell surface proteoglycan that plays a crucial role in various cellular processes, including cell growth, differentiation, and signaling pathways. Recent studies have shown that GPC1 is overexpressed in many types of cancer, including pancreatic, breast, and prostate cancer. This overexpression is thought to contribute to the aggressiveness and metastatic potential of these tumors, making GPC1 a potential target for cancer therapy. One of the key functions of GPC1 in cancer is its ability to interact with growth factors and signaling molecules, such as Wnt and Hedgehog, to promote tumor growth and progression. By blocking the interaction between GPC1 and these molecules, researchers believe they can inhibit the growth of cancer cells and potentially prevent metastasis. In addition to its role in promoting cancer progression, GPC1 has also been found to be a promising biomarker for early detection and prognosis of cancer. Researchers have developed novel diagnostic tools that detect circulating GPC1 levels in the blood, allowing for earlier detection of cancer and more accurate monitoring of treatment response.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Flow cytometry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
