| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KVLQT1 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Guinea pig Anti-KCNQ1 Antibody is an antibody targeting KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KVLQT1 Polyclonal raised in Guinea Pig (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KVLQT1 (also reported as KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KVLQT1).
- Immunogen/epitope region: Intracellular, C-terminus.
- Homology note: Rat, mouse - 14/16 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Cited use: IHC (literature use does not guarantee performance in every setup).
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
KCNQ1 (KV7.1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5).The channel has been intensively studied since it was found that together with MinK (Isk) it is responsible for the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed, mutations in either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death1-3.Although KV7.1 expression and function have been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues where it is involved in physiological functions such as the regulation of acid secretion in the stomach and Cl- secretion into the colon1-3.
Research relevance and current trends
- Mapping receptor/channel localization across neuronal subtypes and subcellular compartments.
- Linking trafficking or surface expression changes to activity-dependent signaling and plasticity.
- Using KO/KD or blocking-peptide concepts to strengthen antibody-based target assignment.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-PC022.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-PC022; Negative control: BLP-PC022.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
