| Field | Specification |
|---|---|
| Species |
H4 cells are a human neuroglioma cell line derived from the central nervous system. These cells are often utilized in neurological research, particularly in studies focusing on neurobiology and neuropharmacology. H4 cells provide a valuable model for understanding the molecular and cellular mechanisms of gliomas, offering insights into tumor biology, response to therapeutic agents, and the regulation of gene expression within the nervous system.
The H4 cell line is known for its robust use in experiments involving neurotoxicity and neuroprotection, serving as a tool to evaluate the effects of various substances on neuronal cells. Researchers use H4 cells to study the cellular processes involved in neurodegeneration and to screen potential neuroprotective and neuroregenerative compounds. Their consistent growth and maintenance characteristics under laboratory conditions make them a reliable resource for in vitro experiments aimed at elucidating neurological functions and disorders.
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- Protein expression: pGP9.5 postive, NeuN positive, NSE negative
- Isoenzymes: G6PD, B, PGM1, 1-2, PGM3, 1, ES-D, 1, Me-2, 0, AK-1, 1, GLO-1, 2.
- Tumorigenic: No
- Karyotype: modal number = 75. range 45 = 80. Y chromosome present
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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