HDEL Antibody

The HDEL sequence (His-Asp-Glu-Leu) is a C-terminal ER retention signal found in many soluble endoplasmic reticulum (ER) resident proteins. It plays a critical role in maintaining ER homeostasis by ensuring that chaperones and folding enzymes are retained or retrieved from the Golgi apparatus back to the ER.

In neurodegenerative diseases, where ER stress and protein misfolding are prominent pathological features, the HDEL motif is essential for the proper localization and function of ER-resident proteins such as GRP78, GRP94, and protein disulfide isomerases. Disruption of HDEL-mediated retention can impair the unfolded protein response (UPR), exacerbate proteotoxic stress, and contribute to neuronal dysfunction.

Moreover, the efficiency of the HDEL retrieval system may decline with age or under chronic stress, potentially leading to the mislocalization of protective chaperones and increased vulnerability to neurodegeneration. Understanding the dynamics of HDEL-mediated trafficking offers insights into ER stress regulation and may inform therapeutic strategies aimed at enhancing proteostasis in neurodegenerative disorders.

1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cite this product varies by variant:

• SMC-175D — Size: 100 ug: HDEL Antibody (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175D, RRID: AB_10556216)
• SMC-175D-A390 — Size: 100 ug: HDEL Antibody: ATTO 390 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175D-A390, RRID: AB_2698789)
• SMC-175D-A488 — Size: 100 ug: HDEL Antibody: ATTO 488 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175D-A488, RRID: AB_2698790)
• SMC-175D-A594 — Size: 100 ug: HDEL Antibody: ATTO 594 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175D-A594, RRID: AB_2698792)
• SMC-175D-APC — Size: 100 ug: HDEL Antibody: APC (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175D-APC, RRID: AB_2698798)
• SMC-175D-BI — Size: 100 ug: HDEL Antibody: Biotin (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175D-BI, RRID: AB_2698799)
• SMC-175D-FITC — Size: 100 ug: HDEL Antibody: FITC (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175D-FITC, RRID: AB_2698800)
• SMC-175D-HRP — Size: 100 ug: HDEL Antibody: HRP (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175D-HRP, RRID: AB_2698801)
• SMC-175D-PCP — Size: 100 ug: HDEL Antibody: PerCP (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175D-PCP, RRID: AB_2698803)
• SMC-175D-RPE — Size: 100 ug: HDEL Antibody: RPE (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175D-RPE, RRID: AB_2698804)
• SMC-175S — Size: 12 ug: HDEL Antibody (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-175S, RRID: AB_10556216)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.