| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone H2AX / H2AFX |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 115-143 from the human protein was used as the immunogen for the Histone H2AX antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Histone H2AX antibody supplied as a purified reagent for IHC-P, WB in Human samples. This product is a monoclonal (mouse origin) antibody (host: Mouse; isotype: IgG1) intended for research use only. The target is commonly annotated with nuclear localization context, which may inform staining patterns.
Key elements and design rationale
- Antibody identity: Monoclonal (mouse origin); host Mouse; isotype IgG1; clone 938CT5.1.1.
- Format and purification: format: Purified; purity: Protein G affinity.
- Species reactivity (reported): Human.
- Applications (listed): IHC-P, WB.
- Immunogen / epitope context: A portion of amino acids 115-143 from the human protein was used as the immunogen for the Histone H2AX antibody..
- Localization: Nuclear (annotation-level guidance; cell state and isoforms can shift patterns).
These attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.
Biological background
Histone H2AX is the intended antigen for this primary antibody. Reported biological context includes: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Subcellular localization information (Nuclear) can be useful when interpreting IF/ICC patterns and selecting compartment-enriched lysates for WB.
Research relevance and current trends
- Post-translational modification mapping: phosphorylation-site–resolved antibodies are used to connect signaling inputs to target activation states and downstream readouts.
- Spatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.
- Interaction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.
Common research applications
- IHC-P: commonly used to measure relative target levels or localization changes in the context of the experimental question.
- Western blot (WB): compare relative abundance/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.
Across these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.
Notes for experimental interpretation
- Isoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.
- Species differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.
- Control concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout/knockdown/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.
Epitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
